What you need to know:
- The mRNA in the vaccine isn’t quite the same as the stuff in your body.
- Before injection, the vaccine is mixed with water containing sodium chloride, or ordinary salt, just as many intravenously delivered drugs are.
Social media has been awash with misleading information on Covid-19 and vaccines. The public, therefore, is entitled to be educated on what constitutes various vaccines to ensure an informed decision as they prepare for vaccination against the deadly virus. The Pfizer vaccine is composed of the following ingredients:
The mRNA: Pfizer’s vaccine is the first on the market that consists of actual genetic information from a virus in the form of messenger RNA, or mRNA, a type of molecule whose usual job is to transport copies of genetic instructions around a cell to guide the assembly of proteins. Imagine an mRNA as a long ticker tape carrying instructions. It’s fairly delicate stuff, and that’s why Pfizer’s vaccine needs to be stored at around -100 °F (-73 °C).
Delivered as a shot in the arm muscle, the vaccine contains an RNA sequence taken from the virus itself; it causes cells to manufacture the big “spike” protein of the coronavirus, which the pathogen uses to glom onto a person’s cells and gain entry. On its own, without the rest of the virus, the spike is pretty harmless. But your body still reacts to it. This is what leaves you immunised and ready to repel the real virus if it turns up.
The mRNA in the vaccine isn’t quite the same as the stuff in your body. That’s good, because a cell is full of defences ready to chop up RNA, especially any that doesn’t belong there. To avoid that, what’s known as “modified nucleosides” have been substituted for some of the mRNA building blocks.
The lipids: The Pfizer vaccine, like one from Moderna, uses lipid nanoparticles to encase the RNA. The nanoparticles are, basically, tiny greasy spheres that protect the mRNA and help it slide inside cells. These particles are probably around 100 nanometers across. Curiously, that’s about the same size as the coronavirus itself. Pfizer says it uses four different lipids in a “defined ratio”. The lipid ALC-0315 is the primary ingredient in the formulation. That’s because it’s ionizable—it can be given a positive charge, and since the RNA has a negative one, they stick together. It's also a component that can cause side-effects or allergic reactions. The other lipids, one of which is the familiar molecule cholesterol, are “helpers” that give structural integrity to the nanoparticles or stop them from clumping. During manufacturing, the RNA and the lipids are stirred into a bubbly mix to form what the FDA describes as a “white to off-white” frozen liquid.
Salts: The Pfizer vaccine contains four salts, one of which is ordinary table salt. Together, these salts are better known as phosphate-buffered saline, or PBS, a very common ingredient that keeps the pH, or acidity, of the vaccine close to that of a person’s body. You’ll understand how important that is if you’ve ever squeezed lemon juice on a cut. Substances with the wrong acidity can injure cells or get quickly degraded.
Sugar: The vaccine includes plain old sugar, also called sucrose. It’s acting here as a cryoprotectant to safeguard the nanoparticles when they’re frozen and stop them from sticking together.
Saline solution: Before injection, the vaccine is mixed with water containing sodium chloride, or ordinary salt, just as many intravenously delivered drugs are. Again, the idea is that the injection should more or less match the salt content of the blood.
No preservatives: Pfizer makes a point of saying its mixture of lipid nanoparticles and mRNA is “preservative-free.” That’s because a preservative that’s been used in other vaccines, thimerosal (which contains mercury and is there to kill any bacteria that might contaminate a vial), has been at the center of worries over whether vaccines cause autism.
There is no thimerosal—or any other preservative—in the Pfizer vaccine. No microchips, either. The vaccine is still known by the code name BTN162, but once it’s authorised, expect Pfizer to give it a new, commercial name that conveys something about what’s in it and what it promises for the world.
Dr P.M Mutua, Immunologist, Makueni